Quantitative Image Analysis and Inverse Modeling of Cell Division
Speaker: Mark Bathe , Laboratory for Computational Cell Biology and Biophysics, Department of Biological Engineering, MIT
Date: May 15 2009
Time: 2:00PM to 3:00PM
Location: 32-D507
Host: Polina Golland, CSAIL
Contact: Polina Golland, x38005, polina@csail.mit.edu
Relevant URL: Cytoskeletal protein dynamics play a central role in various stages of
animal cell division including chromosome congression, spindle-assembly,
chromosome segregation, and cytokinesis. Here, I present our preliminary
efforts to infer mechanistic models of cytoskeletal protein function from
live-cell time-lapse fluorescence imaging. I discuss our computational
approach, which includes spatiotemporal image correlation spectroscopy and
finite element methods, as well as our experimental toolbox that includes
specific protein labeling technology and targeted protein perturbation using
drugs and RNAi. An outlook towards the integration of such mechanistic
models with proteome- and genome-level data that assay gene function,
protein concentrations and phoshporylation states, and protein-protein and
genetic interactions is discussed.
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